Diverse somatic genomic alterations in single neurons in chronic traumatic encephalopathy

Dong, Guanlan, Chanthia C. Ma, Shulin Mao, Samuel M. Naik, Katherine Sun-Mi Brown, Gannon A. McDonough, Junho Kim, et al. 2025. “Diverse Somatic Genomic Alterations in Single Neurons in Chronic Traumatic Encephalopathy”. BioRxiv, Accepted.

Abstract

Chronic traumatic encephalopathy (CTE) is a neurodegenerative disease that is linked to exposure to repetitive head impacts (RHI), yet little is known about its pathogenesis. Applying two single-cell whole-genome sequencing methods to hundreds of neurons from prefrontal cortex of 15 individuals with CTE, and 4 with RHI without CTE, revealed increased somatic single-nucleotide variants in CTE, resembling a pattern previously reported in Alzheimer’s disease (AD). Furthermore, we discovered remarkably high burdens of somatic small insertions and deletions in a subset of CTE individuals, resembling a known pattern, ID4, also found in AD. Our results suggest that neurons in CTE experience stereotyped mutational processes shared with AD; the absence of similar changes in RHI neurons without CTE suggests that CTE involves mechanisms beyond RHI alone.

Last updated on 03/07/2025